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IJE Advance Access originally published online on February 8, 2008
International Journal of Epidemiology 2008 37(Supplement 1):i7-i10; doi:10.1093/ije/dym278
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Published by Oxford University Press on behalf of the International Epidemiological Association © The Author 2008; all rights reserved.

The quality of DNA extracted from liquid or dried blood is not adversely affected by storage at 4°C for up to 24 h

Antony Halsall1, Paul Ravetto2, Yancy Reyes2, Nicola Thelwell2, Alice Davidson3, Rupert Gaut2 and Stephen Little2,*

1Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester M1 7DN, UK.
2DxS Ltd, Manchester, M13 9XX UK.
3Centre for Molecular Medicine, The University of Manchester Stopford Building, M13 9PT UK.

* Corresponding author: Stephen Little, 48 Grafton St, Manchester, M13 9XX, UK. E-mail: stephen.little{at}dxsgenotyping.com


  Abstract

Background A consistent and stable source of DNA is an essential requirement for many Biobanks. An important pre-analytical variable is the delay between sample collection and sample processing.

Methods Fresh blood samples (n = 80) were collected and either processed immediately or after storage for 24 h. The samples were either stored as liquid blood at 4° C or as dried blood spots at ambient temperature on three types of paper-based storage media. The quality of the DNA extracted from the samples was measured.

Results No difference was observed between fresh and stored blood samples.

Conclusions The quality of DNA extracted from liquid or dried blood is not adversely affected by storage at 4° C for up to 24 h.

Keywords DNA, Biobank, storage, dried blood, genetic analysis


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