Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by SIMHON, A
Right arrow Articles by MORAG, A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SIMHON, A
Right arrow Articles by MORAG, A
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1990 Oxford University Press

research-article

How to Predict the Immune Status of Poliovirus Vaccinees? A Comparison of Virus Neutralization at a Very Low Serum Dilution versus ELISA in a Cohort of Infants

A SIMHON*, A LIFSHITZ*, Y ABED**, E E LASCH**, B SCHOUB{dagger} and A MORAG*

*Unit of Clinical Virology, Department of Clinical Microbiology Hadassah University Hospital Jerusalem, Israel.
**Centre for Primary Health Care Gaza.
{dagger}National Institute for Virology Sandringham, South Africa.

Simhon A (Unit of Clinical Virology, Department of Clinical Microbiology, Hadassah University Hospital, Jerusalem, Israel), Lifshitz A, Abed Y, Lasch EE, Schoub Band Morag A. How to predict the immune status of poliovirus vaccinees? A comparison of virus neutralization at a very low serum dilution versus ELISA in a cohort of infants. International Journal of Epidemiology, 1990, 19: 164–168.

A cohort of children from Gaza was observed from birth to the age of one year. Blood specimens were collected at birth, before and after poliovirus vaccination and at one year of age. Poliovirus immunity before and after vaccination was assessed by ELISA and virus neutralization (NT). Positive predictive values for ELISA were between 81.5% and 90.8%. However, ELISA revealed a high frequency of false negatives, and unacceptably low negative predictive values between 28.6% and 55.4%.

The history of poliovirus immunity in the cohort was further investigated by NT. A high level of seropositivity to poliovirus type 1 (PV-1) was found. In cord blood, 83.3% had a NT titre ≥4 and 99.0% had a titre ≥2. Similarly, by one year of age, 85.7% had a titre ≥4 and 90.5% had a titre ≥2. Seropositivity to PV-2 and PV-3 were slightly lower, ie 80.8% of children had a PV-2 titre ≥4and 75.4% had a PV-3 titre ≥4. As for other developing areas, poliomyelitis eradication in Gaza will come about when universal vaccination fills all ‘immunity gaps’ and improved sanitation and housing reduces the endemicity of wild polioviruses.

Revised 1 May 1989


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.